ClonExpress UItra One Step Cloning kit (Vazyme, #C115) is a novel cloning kit, applicable for homologous recombination with 1 - 5 fragments. The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer included in 2x ClonExpress Mix significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.
ClonExpress One Step Cloning is simple, fast, and highly efficient cloning technology which enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp - 20 bp on both 5’- and 3’-end, respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase at 50°C for 5 - 15 min.Product Manual (pdf)
Advantages
- Easy: one-tube super mix.
- Fast: cloning within 5 - 15 min.
- Efficient cloning of fragments of 50 bp-10 kb, with positive clone rate > 95%. Ligase-independent.
- Directional cloning at any site on any vector, no limits in restriction enzyme choice.
- Suitable for cloning of both single and multiple DNA fragments.
When order, Cold Shipping option have to be selected, or your order may be delayed.
Selected Product Citations
- Xing Y, et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell, 2017, 169(4):664-78. Pubmed ID:28475895
- Wu N, et al. TBX6 null variants and a common hypomorphic allele in congenital scoliosis. N Engl J Med, 2015, 4793:341-50. Pubmed ID:25564734
- Ge J, et al. Architecture of the mammalian mechanosensitive Piezo1 channel. . Pubmed ID: Nature, 2015, 527(7576):64-9. Pubmed ID:26390154
- Zhang XO, et al. Complementary Sequence-Mediated Exon Circularization. Cell, 2014, 159(1):134-47. Pubmed ID:25242744
- Zong Y, et al. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature Biotechnology, 2018. Pubmed ID:30272679
- Li X, Wang Y, Liu Y, et al. Base editing with a Cpf1-cytidine deaminase fusion. Nature Biotechnology, 2018, 36(4):324-7. Pubmed ID:29553573
Mechanism
Storage
All components should be Store at -20°C. Avoid repeated freeze and thaw.
Components
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