ChamQ Universal SYBR® qPCR Master Mix (Vazyme, #Q711), protected by Champagne Taq DNA Polymerase (Vazyme, #P122) via an antibody-modified hot-start activation technique, is specially designed for SYBR Green I based quantitative PCR (qPCR). Unique factors (i.e. the specificity-promoting Exactor) in the optimized buffer system of ChamQ Universal SYBR® qPCR Master Mix significantly improve its sensitivity and specificity. This kit contains unique ROX Passive Reference Dye and is applicable for all Real-time PCR instruments, including instrument that needs no ROX dye, low ROX dye, or high ROX dye. The mix is prepared at 2× reaction concentration and can be directly used for robust and low-template qPCR with high sensitivity, specificity, and reliability.Product Manual (pdf)

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Advantages

• Best combination of specificity + sensitivity.
• Unique antibody-modified hot-start taq, with unique specificity-promoting factors.
• Optimal concentrations of Mg2+ and dye.
• Universal: contains a unique ROX Passive Reference Dye and is applicable for all Real-time PCR instruments (no ROX, low ROX, and high ROX).

When order, Cold Shipping option have to be selected, or your order may be delayed.

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Selected Product Citations

1. Guo C, et al. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity, 2018, 49(5): 842-56.. Pubmed ID:30366764
2. Xu L, et al. The transcription factor TCF-1 initiates the differentiation of T(FH) cells during acute viral infection. Nature Immunology., 2015. 16(9): 991-99.. Pubmed ID:26214740
3. Liu X, et al. BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3. Gastroenterology, 2017, 153(6):1607-20.. Pubmed ID:28780076
4. Qiu W, et al. Deciphering Pancreatic Islet β Cell and α Cell Maturation Pathways and Characteristic Features at the Single-Cell Level. Cell Metabolism, 2017, 25(5):1194-205.. Pubmed ID:28467935
5. Zhou H, et al. In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nature Neuroscience, 2018. 21(3):440-6.. Pubmed ID:29335603

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Mechanism

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Storage

All components should be Store at -20°C. Protected from light.

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Components

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Cellagen Technology 公司位于美国加州San Diego，该公司致力于干细胞相关产品的研发和生产。基于多年为世界*的生物公司提供OEM产品的经验，目前Cellagen Technology公司利用自身优势，拓展了原有产品线，推出了以StemRegenin 1（SR1）为代表的多种自有品牌产品。 NamePriceCat # （Powder）Cat # （10 mM in DMSO）Pathway/Target7,8-Dihydroxyflavone1173C7181-10N/ATrkBA7696622023C2769-10N/AAMPKA83-011513C2831-2C2831-2sTGF-β/ALK4,5,7Abiraterone1853C2247-2C2247-2sP450-CYP17BEZ235833C2392-2C2392-2sPI3K, mTORBIX-012941513C2491-5C2491-5sEpigenetics/G9a HMTCHIR990213553C2447-2C2447-2sGSK3β CP-6905501343C2716-2C2716-2sJAK1,2,3Cyclopamine2193C2925-2C2925-2sHedgehog/SmoDAPT1853C3178-5C3178-5sNotch/γ-secretaseForskolin1853C3677-10N/AAdenylate cyclaseFTY7201173C3897-5C3897-5sS1P receptorsGDC-09411513C4321-5C4321-5sPI3KHh-Ag1.53043C4412-2C4412-2sHedgehog/SmoINCB0184241853C4622-2C4622-2sJAK1/2IWP-31853BackorderBackorderWntKHS1011853C5471-5C5471-5sNeural differentiationLDE2252363C5332-2C5332-2sHedgehog/SmoLDN-1931893723C5361-2C5361-2sBMP/ALK2,3Leukadherin-1#VALUE!C5385-2C5385-2sCD11b, CD18LY2940022193C5929-5C5929-5sPI3KP7C32363C7723-10N/Aneurogenic, neuroprotectivePD03259013723C7303-2C7303-2sMEK1, MEK2PD03329912193C7306-2C7306-2sCdk4, Cdk6PS481343C7748-5C7748-5sPDK1Pyrintegrin3383C7974-1C7974-1sES cell survivalR4062193C7406-2C7406-2sSykR7883723C7788-5C7788-5sSykRapamycin2193C7272-5C7272-5smTORReversine1173C7383-2C7383-2sA3 Adenosine ReceptorRO49290972703C7649-2C7649-2sNotch/γ-secretaseSB4315421513C7243-5C7243-5sTGF-β/ALK4,5,7SF16703723C7316-2C7316-2spTENSitagliptin1513C7482-5C7482-5sDPP-4SRT17202363C7781-2C7781-2sSIRT1StemRegenin1 （SR1）3723C7710-1C7710-1sAryl hydrocarbon receptorSU54022703C7854-1C7854-1sFGFRThiazovivin3553C8442-1C8442-1sROCKUrsolic Acid1853C8776-10N/ASTAT3VX-6801173C8916-10N/AAurora kinaseWnt-C592703C7641-2C7641-2sWntXAV9392703C9289-2C9289-2sWntY-276321853C9127-2C9127-2sROCKhuman Activin A5423P4228-1031910 µg37383P4228-1002199100 µghuman BMP-4 3723P4267-1021910 µg25483P4267-1001499100 µghuman bFGF 3383P4234-5019950 µg23783P4234-5001399500 µgmouse LIF 3383P6543-1019910 µg23783P6543-1001399100 µghuman Wnt-3a 3723P4968-1021910 µg